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Lens Epithelial Cells Express CD95 and CD95 Ligand Treatment Induces Cell-death and DNA Fragmentation in Vitro

1Hueber A., 2Eichholtz C. D., 3Kociok N., 1Esser J., 1Esser P.,
1Universität zu Köln, Zentrum für Augenheilkunde (Köln)
2Universität zu Köln, Universitäts-Augenklinik (Köln)
3Universität zu Köln, Zentrum für Augenheilkunde, Abteilung für Netzhaut- und Glaskörperchirurgie (Köln)

Purpose: Despite of advances in intraocular lens design and material posterior capsule opacification (PCO) still remains one of the major problems in modern cataract surgery. Therefore the usage of antiproliferative agents has been advocated. CD95 ligand (CD95L, Fas, Apo-1) is a death ligand that triggers apoptosis in susceptible target cells. Apoptosis allows for the safe disposal of cells without damaging the surrounding tissue. The goal of this project was to characterize and to evaluate CD95L induced cell death in cultured lens epithelial cells (LEC).
Method: Expression of CD95 in untreated porcine LEC was investigated by flow cytometry. Cell death after CD95L or CD95 agonistic antibody treatment was assessed by crystal violet assay and DNA fragmentation was measured by comet assay.
Results: The presence of CD95 was observed in LEC. CD95L treatment resulted in a time and concentration dependent killing of LEC, which was synergistically enhanced by the addition of cyclohexamide (CHX). CD95L treatment induced DNA fragmentation.
Conclusions: The present study confirms the use of apoptosis inducing substances in the inhibition of LEC proliferation. Coating of intraocular lenses with such substances may demonstrate a feasible way of inhibiting PCO.